Regulatory

Part:BBa_K4673025

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)


The FadR regulator regulates the fad gene

FadR regulator controls fatty acid metabolism. In the pET-22b-LK vector, the FadR binding site of the FadH promoter is cloned in front of the lumbrokinase gene (BBa_K4673017), replacing the lac operator. In the absence of fatty acids, FadR will bind to the FadR binding site and inhibit lumbrokinase expression; however, when fatty acids are present, they are degraded into acetyl-CoA by E. coli. This causes acetyl-CoA to bind on and release FadR, allowing lumbrokinase expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None